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Fig. 5 | Molecular Medicine

Fig. 5

From: Clinically relevant pseudoexons of the GALNS gene and their antisense-based correction

Fig. 5

Testing of antisense molecules against PE-2 and PE-5. a,b—schemes of the genomic loci incorporated into minigene vectors and representative gel electrophoresis images of the minigene-specific splicing products are shown for each PE. PEs with adjacent 50 bp are enlarged to indicate the locations of target sites for modU7snRNAs and circRNAs. The relative amounts of transcripts are represented as stacked bar plot with means and standard deviations of three biological replicates. For clarity, maximum of three predominant transcripts are shown on the plot, and the rest are summed up and named “Other”. The PE-containing transcript is colored in dark gray, the correct transcript is colored in green, and the transcript with skipping of the neighbor exon is colored in blue

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